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Salmon Quantifier

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salmon-quantifier

Salmon Quantifier is an Agent Skill for documents published in the a5c-ai/babysitter repository on GitHub: a SKILL.md instruction file an AI agent can load to do this task.

Salmon pseudo-alignment skill for fast and accurate transcript quantification

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How to use this skill

Bring this guide into your coding agent with a prompt tailored to the tool you use.

  1. Open your project in Codex.
  2. Copy and paste the prompt below.
  3. Review files and risks before approving any action.
Prompt to paste
Inspect the Agent Skill at https://github.com/a5c-ai/babysitter/blob/HEAD/library/specializations/domains/science/bioinformatics/skills/salmon-quantifier/SKILL.md and all supporting files. Report provenance, permissions, scripts, dependencies, and security risks. Propose an exact installation into .agents/skills/salmon-quantifier, but wait for my approval before writing files or running scripts.

Copying this prompt does not install or run the skill. Review third-party files before use.

Salmon Quantifier Skill

Purpose

Enable Salmon pseudo-alignment for fast and accurate transcript quantification.

Capabilities

  • Selective alignment mode
  • GC bias correction
  • Mapping rate assessment
  • Bootstrap uncertainty estimation
  • Multi-mapping resolution
  • Decoy-aware indexing

Usage Guidelines

  • Build decoy-aware indices for accuracy
  • Enable GC bias correction
  • Use selective alignment for improved accuracy
  • Generate bootstraps for uncertainty estimation
  • Validate mapping rates against expectations
  • Document index and parameter versions

Dependencies

  • Salmon
  • kallisto
  • RSEM

Process Integration

  • RNA-seq Differential Expression Analysis (rnaseq-differential-expression)
  • Single-Cell RNA-seq Analysis (scrnaseq-analysis)